Journal: NPJ Vaccines

Article Title: Immunization with full-length Plasmodium falciparum merozoite surface protein 1 is safe and elicits functional cytophilic antibodies in a randomized first-in-human trial

doi: 10.1038/s41541-020-0160-2

Figure Lengend Snippet: Sera from vaccinees (1:1000), two malaria semi-immune adults from Burkina Faso (1:1000), and two MSP1 immunized rabbits (1:10,000) were applied to a custom-made MSP1 peptide microarray chip consisting of 1706 oligomeric peptides of 15 amino acids with a peptide-to-peptide overlap of 14 amino acids. Each peptide was printed in duplicate on the chip. As secondary antibodies the appropriate DyLight680 conjugated anti-IgG (Fc) antibodies were used. The fluorescence intensity landscapes across MSP1 are shown for each sample. Relevant epitopes have been highlighted. Controls comprise the placebo and the GLA-SE vaccinees. For orientation, the structural organization of MSP1 is shown below the fluorescence profiles. Full details of epitopes and fluorescence intensities are provided in the Supplementary Table .

Article Snippet: After blocking a peptide microarray copy for 30 min with blocking buffer (Rockland MB-070), pre-staining was done with the respective secondary antibodies goat anti-human IgG (Fc) DyLight680 (0.1 μg ml −1 ), goat anti-human IgM (μ chain) DyLight800 (0.2 μg/ml), anti-rabbit IgG (Fc) DyLight680 (0.2 μg ml −1 ), and the monoclonal mouse anti-HA control antibody (12CA5) DyLight800 (0.5 μg ml −1 ) in incubation buffer for 45 min to investigate background interactions with the printed peptides that could interfere with the main assays.

Techniques: Peptide Microarray, Fluorescence